The tests revealed it is awaiting the results of 250 retests from the 2012 Olympics in London.
"All these measures are a powerful strike against the cheats we do not allow to win," IOC president Thomas Bach said.
"They
show once again that dopers have no place to hide. We keep samples for
10 years so that the cheats know that they can never rest.
"By
stopping so many doped athletes from participating in Rio, we are
showing once more our determination to protect the integrity of the
Olympic competition."
More than 4,500 tests were carried out at the Beijing Games in 2008 but just nine athletes were caught cheating.
The IOC said the retests were focused on athletes who could potentially take part in Rio.
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